abstract：:We have studied the regulation of the human beta-globin gene after retroviral transfer into a variety of transformed and normal hematopoietic cells. After transfer into murine erythroleukemia cells (MEL) expression from the human beta-globin gene responds to inducers of erythroid maturation in parallel to the endogeno...

journal_title：Advances in experimental medicine and biology

authors： Gelinas RE,Bender MA,Miller AD,Novak U

更新日期：1989-01-01 00:00:00

abstract：:Because homeobox-containing genes play a major role in embryogenesis and tissue identity in Drosophila and because similar genes encode tissue-specific transcription factors in mammalian cells, we hypothesized that homeobox genes might plan a role in hematopoietic differentiation and lineage commitment. We therefore s...

journal_title：Advances in experimental medicine and biology

authors： Shen WF,Largman C,Lowney P,Hack FM,Lawrence HJ

更新日期：1989-01-01 00:00:00

abstract：:Storage of fluorescent products, ceroid-like pigments was observed in P388D1 cells, an established macrophage-like cell line, when the cells were cultured in the presence of rat liver phosphatidylcholine liposomes containing polyunsaturated fatty acids. Some fluorescent products accumulated in the cells were extractab...

journal_title：Advances in experimental medicine and biology

authors： Shimasaki H,Maeba R,Ueta N

更新日期：1989-01-01 00:00:00

abstract：:The effects of IABP on the contractile state of the in situ canine heart was evaluated under control conditions and conditions of regional ischemia by determining the transient slope of the end-systolic pressure-volume line, T-Emax. For comparison, the conventional index reflecting contractility, dP/dtmax and other re...

journal_title：Advances in experimental medicine and biology

authors： Chen J,Jiang YS,Fang FZ,Zeng J,Zhao M,Zhang WL,Zhang LF

更新日期：1989-01-01 00:00:00

abstract：:There is a considerable body of evidence indicating that oxidative stress is a causal factor both in lipofuscinogenesis as well as in aging. Studies on the effects of pro-oxidants and antioxidants on lipofuscin accumulation in cultured rat cardiac myocytes and human glial cells indicated that pro-oxidants accelerate w...

journal_title：Advances in experimental medicine and biology

authors： Sohal RS,Brunk UT

更新日期：1989-01-01 00:00:00

abstract：:SFHS modified by covalent incorporation of pyridoxal phosphate using NaCNBH3 as reducing agent had P50 values of 21-25mm Hg, and was insensitive to the addition of BPG. The Hill slope of the modified product was 2.34, demonstrating good functional cooperativity of the subunits. Oxygen carrying capacity, calculated as ...

journal_title：Advances in experimental medicine and biology

authors： Mauldin D,Dalpé RL,Anderson PJ,Biro GP

更新日期：1989-01-01 00:00:00

abstract：:The primary goal of this project was to elucidate the spatial pattern of capillaries in three hamster striated muscles according to statistical techniques of pattern analysis. The spatial pattern of capillaries and traditional measures of capillarity are important to understanding the supply and distribution of oxygen...

journal_title：Advances in experimental medicine and biology

authors： Bennett RA,Pittman RN,Sullivan SM

更新日期：1989-01-01 00:00:00

abstract：:This study was undertaken to further clarify the role of kallikrein-kinin and renin-angiotensin systems in the hypotensive mechanisms of the angiotensin-I converting enzyme inhibitor by using highly sensitive and specific radioimmunoassays in patients with essential hypertension. Captopril was administered for 14 days...

journal_title：Advances in experimental medicine and biology

authors： Iimura O,Shimamoto K

更新日期：1989-01-01 00:00:00

abstract：:A monoclonal antibody (mAb B7C9) to human factor XII was produced which blocked the surface mediated coagulant activity of factor XII but not the amidolysis of H-D-ProPheArg-pNA. The mAb epitope was localized to a 20 amino acid sequence of 2.5 kDa in the heavy chain of factor XII. ...

journal_title：Advances in experimental medicine and biology

authors： Pixley RA,Colman RW

更新日期：1989-01-01 00:00:00

abstract：:Angiotensin-converting enzyme (ACE) activity in the intestinal whole homogenate was showed as three peaks on a column of Sephacryl S-300 HR gel filteration. Over 90% of total ACE activity was found in a soluble fraction separated with an ultracentrifuge of the intestinal homogenate, and the ACE activities were detecte...

journal_title：Advances in experimental medicine and biology

authors： Hayakari M,Amano K,Izumi H,Murakami S

更新日期：1989-01-01 00:00:00

abstract：:In summary, we present a list of phage clones we have obtained from rat genomic libraries (from lamba SC1 to lambda SC31, lambda WC1 and lambda WC40) together with cDNA clones we have obtained from a rat brain cDNA library (PRCM1,5,3 and 4). pRCM5 corresponds to 4.0 kb mRNA species observed primarily in skeletal muscl...

journal_title：Advances in experimental medicine and biology

authors： Nojima H,Sokabe H

更新日期：1989-01-01 00:00:00

abstract：:[Hydroxyproline3]Lysyl-bradykinin ([Hyp3]Lys-BK), a new kinin was isolated, besides Lysyl-bradykinin (Lys-BK), from the reaction mixture of human plasma protein Cohn's fraction IV-4 with hog pancreatic kallikrein. The liberated kinins were isolated by procedures including ethanol extraction, Sephadex G-15, CM cellulos...

journal_title：Advances in experimental medicine and biology

authors： Sasaguri M,Ikeda M,Ideishi M,Arakawa K

更新日期：1989-01-01 00:00:00

abstract：:The components of the kallikrein kinin system are present in brain, and blood pressure is increased when kinins are injected into the brain cerebral ventricles. Therefore, brain kinins may play a role in the regulation of blood pressure. Kinin analogs which are effective as blockers of the vasodilatory and musculotrop...

journal_title：Advances in experimental medicine and biology

authors： Yang XP,Carretero OA,Akahoshi M,Scicli AG

更新日期：1989-01-01 00:00:00

abstract：:Acid proteinase of granulomatous tissues in rats with carrageenin-induced inflammation released two types of kinin from T-kininogen. The kinin was identified as Ile-Ser-bradykinin (T-kinin) and a novel kinin, Met-Ile-Ser-bradykinin (Met-T-kinin), from determination of its amino acid composition and its immunoreactivit...

journal_title：Advances in experimental medicine and biology

authors： Sakamoto W,Satoh F,Nagasawa S,Handa H,Suzuki S,Uehara S,Hirayama A

更新日期：1989-01-01 00:00:00

abstract：:It is well known that patients who undergo surgical operations have a high risk of infection and sepsis. One explanation for this high risk may be a depression of neutrophil functions at the postoperative period. In the present study, the effects of surgical stresses on neutrophil functions were studied in ten patient...

journal_title：Advances in experimental medicine and biology

authors： Utoh J,Yamamoto T,Utsunomiya T,Kambara T,Goto H,Miyauchi Y

更新日期：1989-01-01 00:00:00

abstract：:A quadruple wavelength method to monitor the changes in concentration of oxygenated and deoxygenated hemoglobin and the redox state of cytochrome oxidase within a living tissue is presented. The expected advantages of this technique over the triple wavelength method are (i) that it can compensate for the light scatter...

journal_title：Advances in experimental medicine and biology

authors： Hazeki O,Tamura M

更新日期：1989-01-01 00:00:00

abstract：:Biodegradable and biocompatible microspheres have been investigated for their usefulness as a vaccine delivery system for both parenteral and enteral immunization. Microspheres composed of poly(DL-lactide-co-glycolide) which contained a toxoid vaccine of Staphylococcal enterotoxin B were found to strongly potentiate t...

journal_title：Advances in experimental medicine and biology

authors： Eldridge JH,Meulbroek JA,Staas JK,Tice TR,Gilley RM

更新日期：1989-01-01 00:00:00

abstract：:We studied urinary excretion of active and inactive kallikrein every day for 3 weeks in spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) subjected to 5/6 nephrectomy (5/6), 1/2-nephrectomy (1/2) or sham-operation (Sham). We determined urinary active and inactive kallikrein by measuring kallikrein acti...

journal_title：Advances in experimental medicine and biology

authors： Kanazawa M,Abe K,Yasujima M,Yoshida K,Kohzuki M,Tanno M,Kasai Y,Omata K,Sato M,Takeuchi K

更新日期：1989-01-01 00:00:00

abstract：:Immunohistochemical localizations of calcineurin subunits A (60 KDa) and B (20 KDa) were examined in a rat brain using subunit specific monoclonal antibodies. The immunoreactivity of the subunit A was abundant in the hippocampus, in the striatum, and in the thalamus, but weak in the neocortex. On the contrary, the imm...

journal_title：Advances in experimental medicine and biology

authors： Matsui H,Itano T,Etoh S,Tokuda M,Wang JH,Hatase O

更新日期：1989-01-01 00:00:00

abstract：:We investigated, with use of a technique of feedback analysis, the dynamic changes in and around the renin-angiotensin system in vivo induced by ACE inhibition. Prolonged studies were performed over a half year on rabbits, which were treated by daily injections of saline or one of the inhibitors as follows: captopril,...

journal_title：Advances in experimental medicine and biology

authors： Wada T,Aoyagi T

更新日期：1989-01-01 00:00:00

abstract：:A series of experiments compared the uptake of 5,10,15,20 tetrakis(4-carboxyphenyl) porphinato [67Cu] copper (II), 67CuTCPP, by the lymph nodes of inflamed and two sets or control rats. The results demonstrate that 67CuTCPP localizes in greater concentration in inflamed lymph nodes than in noninflamed control lymph no...

journal_title：Advances in experimental medicine and biology

authors： Cole DA,Mercer-Smith JA,Norman JK,Schreyer SA,Bullington KP,Roberts JC,Lavallee DK

更新日期：1989-01-01 00:00:00

abstract：:Porphyrins form extremely stable chelates with Cu2+. Two copper radionuclides, 67Cu and 64Cu, have attractive nuclear decay properties for use in nuclear medicine applications. We have investigated the use of radiocopper-labeled porphyrins for localization in inflamed tissue and for attachment to antibodies for tumor ...

journal_title：Advances in experimental medicine and biology

authors： Mercer-Smith JA,Cole DA,Roberts JC,Lewis D,Behr MJ,Lavallee DK

更新日期：1989-01-01 00:00:00

abstract：:1. Immunological quantitation of the HPRT proteins, together with DNA and RNA studies have defined further the heterogeneous nature of HPRT-deficiency in our patients. 2. These studies have dictated possible approaches for further characterisation of the HPRT enzyme in our patients. In the two Lesch-Nyhan patients, bo...

journal_title：Advances in experimental medicine and biology

authors： Gordon RB,Keough DT,Sculley DG,de Jersey J,Emmerson BT,Beacham IR

更新日期：1989-01-01 00:00:00

abstract：:The epithelial cells of the gastrointestinal tract from different species show a very variable expression of blood group active glycosphingolipids. The core saccharide sequences are typical for the species as, for example, type 1 chains (Gal beta 1----3GlcNAc) are found in the small intestine of man, rat, and pig and ...

journal_title：Advances in experimental medicine and biology

authors： Hansson GC

更新日期：1988-01-01 00:00:00

abstract：:In attempts to correlate metastatic potential with specific properties of tumor cells, homogeneous subpopulations, which are endowed with low or high metastatic potential, have been selected from Lewis lung carcinoma (3LL). In particular, since cell surface constituents are possibly involved in the metastatic process,...

journal_title：Advances in experimental medicine and biology

authors： Sacchi A,Falcioni R,Tibursi G,Apollonj Ghetti C

更新日期：1988-01-01 00:00:00

abstract：:Amphotropic retroviral vectors containing either the bacterial neomycin phosphotransferase gene or a mutant dihydrofolate reductase gene (DHFR*) were used to infect canine hematopoietic progenitor cells. Successful transfer and expression of both genes in canine hematopoietic progenitor cells has been achieved as meas...

journal_title：Advances in experimental medicine and biology

authors： Schuening F,Storb R,Nash R,Stead RB,Kwok WW,Miller AD

更新日期：1988-01-01 00:00:00

abstract：:The mechanism of the enhancement and the deficit of isometric force in response to slow length changes in tetanized frog muscle fibers was studied by recording the stiffness changes with sinusoidal vibrations (0.5-1.9 kHz, peak-to-peak amplitude 0.1% of L0). When a tetanized fiber was slowly stretched, the fiber stiff...

journal_title：Advances in experimental medicine and biology

authors： Tsuchiya T,Sugi H

更新日期：1988-01-01 00:00:00

abstract：:In isometric contraction, a high proportion of crossbridges are always in the attached state and crossbridge cycling is slow. During shortening, crossbridges must be entering the detached state at a higher rate, as they come to the end of their working strokes. The size of the population of detached crossbridges will ...

journal_title：Advances in experimental medicine and biology

authors： Huxley HE,Kress M,Faruqi AF,Simmons RM

更新日期：1988-01-01 00:00:00

abstract：:The studies on intact fibers of the frog and fast-twitch skinned fibers of the Syrian hamster suggest the presence of intermediate low-force (weak) and high-force (strong) attachments in the cross-bridge cycle. Our results suggest that the weak and strong attachments may be separately regulated by independent calcium ...

journal_title：Advances in experimental medicine and biology

authors： Babu A,Gulati J

更新日期：1988-01-01 00:00:00

abstract：:Overall, the exchange of gas by the lung is strongly dependent on the blood-gas partition coefficient of that gas and weakly dependent on the molecular weight of the gas. The exchange of very soluble inert gases is dependent on interaction with the airway surface during inspiration and expiration. ...

journal_title：Advances in experimental medicine and biology

authors： Hlastala MP,Ralph DD,Babb AL

更新日期：1988-01-01 00:00:00

abstract：:[1-14C]-Eicosapentaenoic acid (EPA) was incubated with porcine leukocytes. Three polar metabolites were isolated after RP-HPLC separation in addition to pentaene leukotrienes and mono-hydroxy fatty acids. These compounds display U.V. absorbance with U.V. lambda max at 302 nm with shoulders at 289 and 317 nm which were...

journal_title：Advances in experimental medicine and biology

authors： Lam BK,Wong PY

更新日期：1988-01-01 00:00:00

abstract：:The diffusion coefficients of cell membranes of pneumocytes plus endothelial cells, cytosol plus blood plasma, erythrocyte membranes, and hemoglobin solution in erythrocytes were estimated from the fluorometrically measured membrane viscosity. The time course of oxygen partial pressure distribution was numerically cal...

journal_title：Advances in experimental medicine and biology

authors： Koyama T,Araiso T

更新日期：1988-01-01 00:00:00

abstract：:The physiological and psychophysical effects of combined flow resistive loads were studied systematically by using the Multistage Evaluation Scale (MES), determining the number of Just Noticeable Difference (JND) steps, and constructing equivalent resistive-load-sensation contours. During combined resistive loading, t...

journal_title：Advances in experimental medicine and biology

authors： Zhang LF,Wu XY,Jiang SZ,Zhang R,Ma MC

更新日期：1988-01-01 00:00:00

abstract：:In summary, it can be said that in spite of possible limitations, this technique provides previously unattainable data on capillary flow pattern in the myocardium. Qualitative and quantitative results indicate that the following anatomical and physiological concepts should be incorporated in any further modelling of o...

journal_title：Advances in experimental medicine and biology

authors： Reeves WJ,Rakusan K

更新日期：1988-01-01 00:00:00

abstract：:Various members of the respiratory chain exhibit different optical properties in the reduced and oxidized forms, thus enabling the non-invasive monitoring of various organs in vitro as well as in vivo. Since the pioneering work of Chance, Cohen, Jobsis and Schoener in 1962, many groups of investigators adopted their a...

journal_title：Advances in experimental medicine and biology

authors： Mayevsky A,Nioka S,Chance B

更新日期：1988-01-01 00:00:00

abstract：:Our new method is comparable to the standard, "open-end" tonometer system and incorporates all its abilities to monitor oxygen tension and perfusion. The new device has the advantage of being completely sealed, providing a true, continuous direct measurement over several days, with freedom of movement for the patient ...

journal_title：Advances in experimental medicine and biology

authors： Rabkin J,Alena R,Morse J,Goodson WH 3rd,Hunt TK

更新日期：1988-01-01 00:00:00

abstract：:Before any clinical signs of diabetic retinopathy can be detected, two major histopathological lesions occur in the walls of retinal capillaries: basement membrane thickening; and loss of intramural pericytes (mural cells). A decrease in the number of pericyte to endothelial cell junctions also occurs under diabetic c...

journal_title：Advances in experimental medicine and biology

authors： Robison WG Jr

更新日期：1988-01-01 00:00:00

abstract：:New methods have been developed for the preparation of highly purified gangliosides, homogeneous in the saccharide, long chain base, and fatty acid moieties and gangliosides carrying different kinds of labelled probes. Gangliosides, homogeneous in the oligosaccharide portion, were prepared by preparative normal phase ...

journal_title：Advances in experimental medicine and biology

authors： Sonnino S,Ghidoni R,Gazzotti G,Acquotti D,Tettamanti G

更新日期：1988-01-01 00:00:00

abstract：:This paper develops an analysis of the interaction between circulatory convective and tissue diffusive O2 transport that explains how VO2 max is set by the integrated response to each and every step in the O2 transport chain from atmosphere to mitochondria. We suggest that it is not useful to search for "the" rate lim...

journal_title：Advances in experimental medicine and biology

authors： Wagner PD

更新日期：1988-01-01 00:00:00

abstract：:This paper has described an animal (rodent) model of depression in which depression-like characteristics are produced by exposure of animals to stressful events that they cannot control. This model, called "stress-induced depression," appears to be mediated by stress-induced changes in brain norepinephrine (NE), and e...

journal_title：Advances in experimental medicine and biology

authors： Weiss JM,Simson PE

更新日期：1988-01-01 00:00:00